ISSN 1301 - 0883 | E-ISSN: 1309-3886
Eastern Journal of Medicine
Investigation of Rotenone Effects on Ovarian Tissue Using Stereological Methods [Eastern J Med]
Eastern J Med. 2024; 29(1): 81-85 | DOI: 10.5505/ejm.2024.37880

Investigation of Rotenone Effects on Ovarian Tissue Using Stereological Methods

Neşe Çölçimen1, Hülya Özdemir2
1Department of Histology and Embryology,Van Yüzüncü Yıl University, Van, Turkey
2Department of Pharmacology, Van Yüzüncü Yıl University, Van, Turkey

INTRODUCTION: This study aims to stereologically investigate the effects of rotenone on rat ovarian tissue.
METHODS: Within the scope of the study, eighteen female rats were taken and divided into 3 groups as control group, to which physiological saline was administered, vehicle group to which sunflower oil was administered, and rotenone group to which rotenone dissolved in sunflower oil was administered at a dose of 2 mg/kg subcutaneously (s.c.). At the end of the experimental, the right ovarian tissue was excised and removed, and kept in 10% formaldehyde for 72 hours. Then routine histological follow-up process were applied, and the tissues were embedded in paraffin. The sections of 5µm thickness were taken and stained with Haematoxylin-Eosin (H&E) and Masson's trichrome viewed under a light microscope and photographed.
RESULTS: When the histological structure of the ovarian tissue in the rotenone group was examined, it was observed that there was a remarkably decreased in the number of follicles. When the total ovarian tissue volumes of the groups were compared stereologically, it was determined that the total ovarian tissue volume decreased in the Rotenone group and this decrease was statistically significant(p<0.05).
DISCUSSION AND CONCLUSION: As a result, it was determined in our study that Rotenone has harmful effects on the ovary, which is a part of the female reproductive system.

Keywords: : Ovary, Rat, Rotenone, Stereology, Toxicity

Neşe Çölçimen, Hülya Özdemir. Investigation of Rotenone Effects on Ovarian Tissue Using Stereological Methods. Eastern J Med. 2024; 29(1): 81-85

Corresponding Author: Neşe Çölçimen, Türkiye
Manuscript Language: English
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