ISSN 1301 - 0883 | E-ISSN: 1309-3886
Prophylactic effects of silver nanoparticles in a rat model of spinal stabilization and infection [Eastern J Med]
Eastern J Med. 2022; 27(1): 126-132 | DOI: 10.5505/ejm.2022.56254

Prophylactic effects of silver nanoparticles in a rat model of spinal stabilization and infection

Abdurrahim Tas1, Feyza Karagoz Guzey2, Azmi Tufan2, Nuray Kuvat3, Özgür Yusuf Aktaş4, Murat Yücel5, Asli Kahraman Akkalp6
1Yuzuncu Yıl University, School Of Medicine Department Of Neurosurgery Van, Turkey
2Health Sciences University Bağcılar Training Research Hospital Neurosurgery Clınıc İstanbul/Turkey
3Ahenk laboratory Clinical Microbiology Clinic İstanbul/Turkey
4Manisa city hospital Neurosurgery Clınıc Manisa/Turkey
5Sivas Numune Hospital Neurosurgery Clınıc Sivas/Turkey
6Katip Celebi University Ataturk Training and Research Hospital, Department of Pathology Izmir/ Turkey

INTRODUCTION: Several factors increase the risk of postoperative infection. Some experimental studies have shown that silver nanoparticles (AgNP) have a protective effect from surgical infection. This study aimed to determine the effectiveness of AgNP in preventing the development of infection in the spinal stabilization rat model.

METHODS: In this study, 32 Sprague-Dawley rats weighing 300–350 g were used. Subjects were randomly divided into four groups. Titanium screws were placed in the spine in all groups. In Groups 2, 3, and 4, S.aureus and P.aeruginosa were cultivated in the surgical site at a concentration of 106 CFU/10 μL. Additionally, 0.5 mg/kg AgNP in maltose solution in Group 3 and maltose solution in Group 4 were used. The histopathological examinations were then done.

RESULTS: In the fascia, muscle, bone, and screws in all groups, except in Group 1 (control group), S. aureus and P.aeruginosa reproduced. In blood cultures, there was growth of S. aureus and P. aeruginosa in 1 subject in Group 2, 6 subjects in Group 3, and 2 subjects in Group 4. The growth of S. aureus and P.aeruginosa was significantly increased in Group 3. The degree of inflammation was significantly lower in Group 1. No accumulation of silver was detected in any organs histopathologically.

DISCUSSION AND CONCLUSION: Application of 1 eucal AgNP decreased the severity of infection. This dose was not toxic. However, administration of AgNP at this dose did not decrease the local infection rate. Therefore, new studies should be conducted to determine the ideal effective dose.

Keywords: rat, surgical infection, silver nanoparticle, spinal stabilization

Abdurrahim Tas, Feyza Karagoz Guzey, Azmi Tufan, Nuray Kuvat, Özgür Yusuf Aktaş, Murat Yücel, Asli Kahraman Akkalp. Prophylactic effects of silver nanoparticles in a rat model of spinal stabilization and infection. Eastern J Med. 2022; 27(1): 126-132

Corresponding Author: Abdurrahim Tas, Türkiye
Manuscript Language: English
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