The Effect of Indole-3-Acetic Acid on Some Metabolic Enzymes in Kidney of the Second Cross Maternal Mice and Their Offsprings

Indole-3-acetic acid (IAA) generates reactive oxygen species (ROS) and excited oxygen intermediates. IAA causes renal dysfunction, hypoglycemia, and myotonia. The first aim of the study is to investigate the possible indirect effect of the plant growth hormone IAA on some renal enzyme activities such as hexokinase (HK), glucose-6-phosphate dehydrogenase (G6PD), 6-phosphogluconate dehydrogenase (6PGD), lactate dehydrogenase (LDH), and malate dehydrogenase (MDH) of second cross offsprings of the second cross maternal mice (Mus musculus) that are given IAA. The second aim was to investigate the possible direct effect of IAA on the same renal enzymes in second cross maternal mice. Method: Female mice was divided into two groups: IAA administered group and ethanol control group. Two chemicals used were applied intraperitoneally. IAA was administrated to maternal mice as a 1/40 dilution of 300 mg/kg body weights in 3-day intervals. Ethanol was administrated in the controls. Spectrophotometric methods were used to determine the activities of enzymes in the kidney of the second cross-maternal mice and their offsprings. Results: In the second cross-maternal mice, the activities of LDH, MDH, and G6PD were higher in IAA administrated animals than in controls. 6PGD and HK showed decreases in IAA study group compared to control group. In the offsprings of the second cross maternal mice, the activities of G6PD, HK, and MDH enzymes were decreased, and 6PGD and LDH enzymes were increased in IAA group compared to control group. Even so differences were not statistically significant. Conclusion: The results obtained suggested that metabolic enzymes studied were not affected from IAA toxicity. This may be due to low dose of IAA.