ISSN 1301 - 0883 | E-ISSN: 1309-3886
Evaluation of the antiseptic efficacy of 4% chlorhexidine gluconate and 10% povidone iodine on Methicillin-Resistant Staphylococcus Aureus-infected wounds in White Rat (Rattus Norvegicus) [Eastern J Med]
Eastern J Med. 2023; 28(3): 378-387 | DOI: 10.5505/ejm.2023.25901

Evaluation of the antiseptic efficacy of 4% chlorhexidine gluconate and 10% povidone iodine on Methicillin-Resistant Staphylococcus Aureus-infected wounds in White Rat (Rattus Norvegicus)

Fanny Evasari Lesmanawati, Agus santoso Budi, Lobredia Zarasade
Department Of Plastic Reconstructive And Aesthetic Surgery, Universitas Airlangga, Dr Soetomo General Academic Hospital, Surabaya, East Java, Indonesia

INTRODUCTION: Methicillin-resistant Staphylococcus aureus (MRSA), an opportunistic bacterium that harms immunocompromised hosts, was 8.1% at our hospital in 2014, with 8.2% of cases in the surgical wards. Chlorhexidine gluconate (CHG) and povidone iodine (PVP-I) are excellent antiseptics for MRSA-infected wounds.
METHODS: Groups of male Wistar rats (n=15) were wounded and inoculated with MRSA then divided into three groups of intervention; 4% CHG, 10% PVP-I, and saline (control). After a six-hour incubation, each wound was intervened. Swab samples were taken before (first result), after intervention (second result), and 24 hours after intervention (third result), followed by a tissue sample taken in the end (fourth result). Using colony-forming units (CFU)/ml, the bacterial colonies were divided into three logarithmic groups: log10 1, ≤ log10 5 (≠ 0), and > log10 5. All data were analysed using a statistic with a significance level of P < 0.05%.
RESULTS: Only the second result of all groups showed a reduction in colony number, while the other results showed > log10 5 CFU/ml. Sixty percent of the second result of PVP-I group showed no bacteria, the rest, 40%, showed ≤ log10 5 (≠0) CFU/ml. All of the second result of CHG group showed no bacteria. We found a significant difference in the second results of all groups (P = 0.009) and in the comparison between CHG versus control group and PVP-I versus control group (P = 0.003 and 0.049, respectively).
DISCUSSION AND CONCLUSION: The effectiveness of 4% CHG and 10% PVP-I is equivalent in MRSA-infected wound care in this study.

Keywords: wound care, Wistar rats, Rattus novergicus, chlorhexidine gluconate, povidone iodine, methicillin-resistant Staphylococcus aureus, MRSA-infected wound

Corresponding Author: Fanny Evasari Lesmanawati, Indonesia
Manuscript Language: English
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