ISSN 1301 - 0883 | E-ISSN: 1309-3886
Esculetin induce apoptosis in Human Cervical Cancer Cells (HeLa) [Eastern J Med]
Eastern J Med. 2024; 29(1): 18-21 | DOI: 10.5505/ejm.2024.25564

Esculetin induce apoptosis in Human Cervical Cancer Cells (HeLa)

TUĞÇE DURAN1, GOZDE SAHİN2, aysegul kebapcılar3, cetin celik4
1Department Of Medi̇cal Geneti̇cs,kto Karatay Unıversıty Medıcal Faculty,konya,turkey
2Department Of Gynecologıc Oncology,başakşehi̇r Çam And Sakura Cıty Hospi̇tal,i̇stanbul,turkey
3Department Of Gynecology And Obstetrıcs,selcuk Unıversıty Medıcal Faculty,konya,turkey
4Department Of Gynecology And Obstetrics,selcuk University Medical Faculty,konya,turkey

INTRODUCTION: Despite the development of effective HPV screening and vaccination programs in women, cervical cancer ranks first in cancer-related deaths. In order to prevent the side effects of chemotherapeutic agents, studies on the use of natural compounds in the treatment of cervical cancer are becoming increasingly common. Esculetin, a coumarin derivative from natural compounds, is a pharmacological substance with anti-inflammatory, antioxidant, anti-tumor, neuroprotective effects. In various studies, esculetin has been found to induce apoptosis in colon cancer, breast cancer, and cervical cancer via JNK, ERK, ROS mediated mitochondrial pathway or ER stress activation pathways
METHODS: HeLa cells and HEK cells were cultured. MTT analysis was performed to determine the IC50 dose of the esculetin. Expression levels of apoptotic genes (BAX, BAK, BAD, APAF-1 and p53) and antiapoptotic genes (BCL-2, BCL-XL) were detected by qPCR.
RESULTS: The IC50 dose at which esculetin inhibited the viability of HeLa cells was determined as 800 μM.In addition, esculetin down-regulated antiapoptotic genes BCL-2 and BCL-XL, while up-regulated apoptotic genes such as BAK, APAF-1, p53, BAD, BAX.
DISCUSSION AND CONCLUSION: Current findings showed that esculetin has apoptotic effects on HeLa cells.

Keywords: Apoptosis, Cervical cancer, coumarin derivative, HeLa cell

TUĞÇE DURAN, GOZDE SAHİN, aysegul kebapcılar, cetin celik. Esculetin induce apoptosis in Human Cervical Cancer Cells (HeLa). Eastern J Med. 2024; 29(1): 18-21

Corresponding Author: TUĞÇE DURAN, Türkiye
Manuscript Language: English
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