Light microscopic determination of tissue

Tissues to be examined under a light microscope, must pass through certain stages. These are; detection(fixation), washing, dewatering (dehydration), polishing or transparent, absorption, recession or blocking, cutting, coloring and closing the lamella. At the end of this phase examination of the tissues with a light microscope takes place. Mistakes at any stage, may lead to irreparable consequences. For this purpose, the researcher during the examination of biological materials should properly fulfill tissue tracking methods. It is aimed to review the existing information about the first phase of tissue detection and to review and compile new publications. It was aimed to review present knowledge and recent literature on the fixation step which is the first step in tissue determination.