Phenotypic and Genotypic Analysis of the Antibiotic Resistance Profiles of Gram Negative Bacteria Isolated from the Blood Culture Samples

INTRODUCTION: Sepsis, which is common and often lethal, is a serious public health problem. There is consensus that the incidence is increased in patients with sepsis due to an aging population, increased use of immunosuppressive therapy, and high-risk interventions.
METHODS: Seven hundred fifty patients with suspected sepsis hospitalized in intensive care units in the Health Education University Van Training and Research Hospital were evaluated. 750 patients were classified according to their age and sex. Bacteria were isolated from their blood cultures. Biochemical tests such as catalase test, oxidase test and Gram staining were performed. Vitek 2 Compact (Biomerieux, USA) device was used for identification of bacteria and evaluation of the antibiogram test. The blaOXA-48 and blaIMP genes were analyzed by polymerase chain reaction (PCR).
RESULTS: Ten multidrug-resistant bacteria were isolated and identified from patients with sepsis. Of our gram negative bacteria, only 2 K. pneumoniae isolates were found to be blaOXA-48 carriers. No blaIMP gene was found in any of our gram negative bacteria causing sepsis.
DISCUSSION AND CONCLUSION: Ýdentification of the bacteria causing sepsis in our hospital and determining the antibiotic resistance rates were found quite important. Among the causative agents of bloodstream enfections, effect of the presence of carbapenem, enlarged β-lactamase and multidrug resistance bacteria on human health were found to be very important.