ISSN 1301 - 0883 | E-ISSN: 2687-5101
Eastern Journal of Medicine
Herpes simplex encephalitis cases with typical and atypical symptoms confirmed by PCR-amplification of the DNA polymerase gene [Eastern J Med]
Eastern J Med. 2000; 5(2): 70-72

Herpes simplex encephalitis cases with typical and atypical symptoms confirmed by PCR-amplification of the DNA polymerase gene

G.M. Matar0, W.A. Khoudoud0, M. Fayad0, R. Choueiri0, M. Mikati2, A.M. Abdelnoor0
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Objective: We have confirmed by PCR-amplification of the DNA polymerase gene, three cases of Herpes Sim- plex encephalitis (HSE) with atypical clinical symp- toms. Methods: Cerebrospinal fluid (CSF) samples were col- lected by lumbar puncture during the course of test- ing from 23 patients suspected to have viral encepha- litis based on commonly encountered symptoms, cy- tochemical analysis and neurological testing. PCR was done on CSF using specific primers that amplify a 290 base pair sequence on the DNA polymerase gene com- mon to both HSV-1 and HSV-2 strains. Results: Only 3 of the 23 CSF samples collected showed the expected amplicon size on the target se- quence. One case had conventional symptoms of en- cephalitis and 2 cases showed atypical symptoms from the conventional ones. All 3 patients recovered after administration of acyclovir. Fourteen PCR-negative patients were shown to have non-HSE viral encephali- tis and the remaining 6 patients were shown later to have other neurological diseases. Conclusion: PCR was efficient in elucidating the atypi- cal cases of HSV encephalitis.

Keywords: HSV, encephalitis, PCR

G.M. Matar, W.A. Khoudoud, M. Fayad, R. Choueiri, M. Mikati, A.M. Abdelnoor. Herpes simplex encephalitis cases with typical and atypical symptoms confirmed by PCR-amplification of the DNA polymerase gene. Eastern J Med. 2000; 5(2): 70-72
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