INTRODUCTION: In our study we have investigated polymorphism due to base change occurring at -455 position of promoter region of β-fibrinogen synthesizing gene in lung cancer patients. In addition, we have also investigated insertion/deletion (I/D) polymorphism in intron 16 of Angiotensin Converting Enzyme (ACE) gene, located on chromosome 17q23. Aim of our study is to determine polymorphisms of β-fibrinogen -455 G/A and ACE I/D in patients with lung cancer and healthy control and to determine whether these polymorphisms contribute to the formation of lung cancer. In addition, we aimed to clarify whether β-fibrinogen -455 G/A polymorphism effect the level of fibrinogen synthesis in patients with lung cancer.
METHODS: Samples in our study was obtained from 100 patients with lung cancer and 100 healthy volunteers. Plasma fibrinogen levels were measured with ELISA method. Polymorphism analyses were determined with PCR-reverse hybridization method.
RESULTS: Results of our study revealed that fibrinogen levels of patients with lung cancer were significantly higher than healthy control group (p<0.01). No difference was determined for β-fibrinogen -455 G/A and ACE I/D polymorphisms between patients with lung cancer and healthy control group. In both of the groups fibrinogen level was higher in AA genotype compared to GG and GA genotypes in both of the groups (p<0.05)
DISCUSSION AND CONCLUSION: As a result, it can be stated that β-fibrinogen -455 G/A and ACE I/D polymorphisms are not related with formation and prognosis of lung cancer.