ISSN 1301 - 0883 / E-ISSN 1309 - 3886
 
 








  Evaluation of a serological test for the diagnosis of tuberculosis using the 38kda antigen [Eastern J Med]
Eastern J Med. 2000; 5(1): 10-12

Evaluation of a serological test for the diagnosis of tuberculosis using the 38kda antigen

A. M. Abdelnoor0, G. Khaled0, G.M. Matar0, M.N. Khalil0, F. Ramadan0

To evaluate an enzyme immunoassay utilising the 38 KDa Mycobacterial antigen as a possible test for the laboratory diagnosis of tuberculosis. Method: The sera of 37 patients with confirmed tuberculosis, 37 tuberculin-negative, apparently healthy individuals and 27 tuberculin-positive, apparently healthy individuals were tested. Moreover, seropositive, apparently healthy individuals were tested by a peripheralblood- based PCR assay using a broad range bacterial primer and one that is specific for the M. tuberculosis complex. Results: Four of 37 patients with confirmed tuberculosis, 10 of 37 tuberculin-negative, apparently healthy individuals and none (0 of 27) of the tuberculin-positive, apparently healthy individuals were seropositive. All seropositive, apparently healthy individuals were negative by PCR. Conclusion: The sensitivity of the enzyme immunoassay utilising the 38 KDa antigen in this study was 10.8% and the specificity 84.4%. Thus, it appears that this test may not be used for the diagnosis of tuberculosis in the Lebanese.

Keywords: Tuberculosis, enzyme immunoassay, 38 Kda antigen, PCR.


A. M. Abdelnoor, G. Khaled, G.M. Matar, M.N. Khalil, F. Ramadan.  Evaluation of a serological test for the diagnosis of tuberculosis using the 38kda antigen. Eastern J Med. 2000; 5(1): 10-12


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