ISSN 1301 - 0883 / E-ISSN 1339 - 3886
 
 







 
 
Search





Investigation of antibiotic resistance genes of beta-hemolytic and non-hemolytic streptococci with molecular methods [Eastern J Med]
Eastern J Med. 2019; 24(1): 47-52 | DOI: 10.5505/ejm.2019.68542  

Investigation of antibiotic resistance genes of beta-hemolytic and non-hemolytic streptococci with molecular methods

Gülhan Bora1, Ömer Akgül1, Elif Naz Kıymaz2
1Department of Pharmaceutical Microbiology, Faculty of Pharmacy,Yüzüncü Yıl University,VAN, Turkey
2Diğer

INTRODUCTION: Infectious diseases have been one of the most important problems affecting all societies since the existence of mankind. Streptococci are an important cause of diseases in humans such as meningitis, acute otitis media, rhinosinusitis and community-acquired pneumonia.
METHODS: In this study, various clinical samples belonging to the disease were taken between January and September 2015. Analyzes of determined and identified antibiotic resistance genes of isolated and identified streptococci strains were carried out in the Microbiology laboratory of Yüzüncü Yıl University Faculty of Pharmacy by PCR. The detection of pbp1a for penicillin G antibiotic, pbp2x for cefotaxime antibiotic, and gyrA resistance genes for quinolone antibiotics were targeted. In this study, the primers used in Table-2 were taken as reference.
RESULTS: A total of 30 streptococcus strains were isolated and identified from various clinical samples including 8 sputum, 6 urine, 5 BOS, 4 blood, 4 nasopharyngeal swab, 2 ear effusion and 1 abse. As a result of the PCR analysis, the resistance of pbp1a was found in 6 of the streptococcal isolates, pbp2x in 8, and resistance in the gyrA gene region in 5. In our study, no three resistance genes were found in 22 isolates and the presence of all three (pbp1a, pbp2x and gyrA) resistance genes in 5 isolates was determined.
DISCUSSION AND CONCLUSION: It is expected that the data obtained from this research will contribute to national and international knowledge accumulation.

Keywords: Streptococcus, pbp1A, pbp2X, gyrA


Gülhan Bora, Ömer Akgül, Elif Naz Kıymaz. Investigation of antibiotic resistance genes of beta-hemolytic and non-hemolytic streptococci with molecular methods. Eastern J Med. 2019; 24(1): 47-52

Corresponding Author: Ömer Akgül, Türkiye


TOOLS
Full Text PDF
Print
Download citation
RIS
EndNote
BibTex
Medlars
Procite
Reference Manager
Share with email
Share
Send email to author

Similar articles
PubMed
Google Scholar




 
Copyright © 2019 Eastern Journal of Medicine. All Rights Reserved.
Home        |        Contact
LookUs & OnlineMakale