ISSN 1301 - 0883 / E-ISSN 1309 - 3886
 
 








  Do Avanafil and Zaprinast Change Some Selected Cytokine Levels in Ovariectomized Rat's Liver? [Eastern J Med]
Eastern J Med. 2020; 25(3): 383-387 | DOI: 10.5505/ejm.2020.57689  

Do Avanafil and Zaprinast Change Some Selected Cytokine Levels in Ovariectomized Rat's Liver?

Zübeyir Huyut1, Nuri Bakan2, Erdem Çokluk3, Halil İbrahim Akbay1, Hamit Hakan Alp1, Mehmet Ramazan Şekeroğlu1
1Department of Biochemistry, Medical Faculty, Van Yuzuncu Yıl University, Van, Turkey
2Department of Biochemistry, Medical Faculty, Ataturk University, Erzurum, Turkey
3Department of Biochemistry, Medical Faculty, Sakarya University, Sakarya, Turkey

INTRODUCTION: Studies reported that phosphodiesterase-5 inhibitors (PDE-5Is) positively contributed to bone-mineral-density and thickness in rats with ovariectomy, which have the same condition with postmenapozal period. To explain the positive contribution mechanism on bone mineral density of PDE-5Is, we investigated the effect of zaprinast and avanafil on levels of some pro-or anti-resorptive cytokines in ovariectomized-rats.
METHODS: Albino female rats (8 months and 250-350 g) were used and four groups of equal-number were randomly assigned (n=6). Groups; was the sahm operated, positive control (OVX), Zaprinast and OVX, Avanafil and OVX groups, respectively. The levels of Estrogen, IL-1β, IL-6, IL-8, IL-10 and TNF-α were measured by ELISA kits, in liver of rats.
RESULTS: IL-1β, IL-6, IL-8 and TNF-α levels were high in groups with OVX compared to sham group, while IL-10 levels were low. Also, IL-1β, IL-6, IL-8 and TNF-α levels were low in zaprinast and especially avanafil-treated groups with OVX and were similar to the sahm group values (p=0.001 for IL-1β, p=0.045 for IL-6, p=0.008 for IL-8, p=0.006 for IL-10, p=0.026 for TNF-α).
DISCUSSION AND CONCLUSION: Zaprinast and especially avanafil inhibited IL-1β, 8 and TNF-α and increased the IL-10 levels compared to the OVX group. This may support opinion that PDE-5Is enhance bone mineralization by inhibiting proresorptive cytokines.

Keywords: Avanafil, phosphodiesterase-5 inhibitors, cytokine, ovariectomy, zaprinast


Zübeyir Huyut, Nuri Bakan, Erdem Çokluk, Halil İbrahim Akbay, Hamit Hakan Alp, Mehmet Ramazan Şekeroğlu. Do Avanafil and Zaprinast Change Some Selected Cytokine Levels in Ovariectomized Rat's Liver?. Eastern J Med. 2020; 25(3): 383-387

Corresponding Author: Zübeyir Huyut, Türkiye


TOOLS
Full Text PDF
Print
Download citation
RIS
EndNote
BibTex
Medlars
Procite
Reference Manager
Share with email
Share
Send email to author

Similar articles
PubMed
Google Scholar




 
Copyright © 2020 Eastern Journal of Medicine. All Rights Reserved.
Home        |        Contact
LookUs & OnlineMakale