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Lipoic acid decreases 3-nitrotyrosine and cytokine levels in a rat sepsis model [Eastern J Med]
Eastern J Med. Ahead of Print: EJM-14237 | DOI: 10.5505/ejm.2019.14237  

Lipoic acid decreases 3-nitrotyrosine and cytokine levels in a rat sepsis model

Behzat Çimen1, Leyla Çimen2, İhsan Çetin3
1Department of Biochemistry, Erciyes University, Faculty of Pharmacy, Kayseri, Turkey
2Department of Medical Biochemistry, Erciyes University, Faculty of Medicine, Kayseri, Turkey
3Department of Medical Biochemistry, Hitit University, Faculty of Medicine, Çorum, Turkey

INTRODUCTION: It was aimed to investigate the effects of lipoic acid (LA) on 3-nitrotyrosine (3-NT), interleukin-1β (IL-1β), tumor necrosis factor- α (TNF-α), magnesium and trace elements levels in serum of rats with sepsis.
METHODS: Control group (n=10) received a single dose of saline, lipopolysaccharide (LPS) group (n=10) was injected a single dose of 20 mg/kg LPS, and LA+LPS (n=10) group was injected a single dose of 20 mg/kg LPS at the end of third day of 10 mg/kg/day LA injection. The serum 3-NT levels were determined by a high performance liquid chromatography. The serum levels of IL-1β and TNF-α were measured by enzyme linked immunosorbent assay. Determination of magnesium and trace element levels were performed by inductively coupled plasma mass spectrometer.
RESULTS: 3-NT, IL-1β and TNF-α levels in control and LA+LPS groups were not determined. However, observable levels of these parameters were detected in the LPS group. It was found that manganese levels were positively correlated with IL-1β and TNF-α in LPS group. LA increased the manganese levels, while it decreased magnesium and iron levels in LA+LPS group.
DISCUSSION AND CONCLUSION: Our results show that LA decreases serum levels of 3-NT, IL-1β and TNF-α in rats with sepsis, and it is also effective on trace element levels. Decreased levels of manganese and its association with IL-1β and TNF-α in the LPS group suggest that manganese may be associated with immune response.

Keywords: Sepsis, lipoic acid, 3-nitrotyrosine, tumor necrosis factor-alpha, interleukin-1β,,




Corresponding Author: İhsan Çetin, Türkiye


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