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In vivo comparative immunotoxic study of histamine receptors (H1R, H2R, H3R and H4R)-agonist [Eastern J Med]
Eastern J Med. 2010; 15(2): 48-56

In vivo comparative immunotoxic study of histamine receptors (H1R, H2R, H3R and H4R)-agonist

Trivendra Tripathi1, Mohammad Shahid2, Adil Raza2, Haris M. Khan3, Mashiatullah Siddiqui1, Abida Malik3, Rahat Ali Khan4
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Accumulating evidences have highlighted histamine and histamine receptors (HRs)-antagonists’ role in immunomodulation. However, the roles of HRs-agonists are still unclear. The present study was therefore designed to delineate the comparative immunotoxic roles of H1-H4-agonist on antibody generation profile in rabbit model. The cohort comprised of seven groups (Group-I negative control, group-II positive control and group-III-VII HRs-agonist-treated) containing 18 (9 male and 9 female) rabbits each. Group-I and group-II received vehicle (sterile distilled water, 1mlkg-1×b.i.d.) intramuscularly. Groups-III-VII (HRs-agonist-treated) received subcutaneous histamine (100µgkg-1) and H1-agonist (HTMT), H2-agonist (amthamine), H3-agonist (R-[-]-?-methylhistamine) and H4-agonist (clobenpropit) each in a dose of 10µgkg-1, respectively, b.i.d. for 10 days (starting from day 1). Groups-II-VII were subsequently immunized with intravenous injection of SRBC at day 3. The estimation of serum immunoglobulins (Ig), IgM and IgG were done by ELISA, and observed at day 0 (pre-immunization) and day 7, 14, 21, 28 and 58 (post-immunization). Results showed that histamine and HRs-agonist could influence a detectable antibody response to SRBC as early as day 7-postimmunization (post-I), which lasted until day 58- post-I. All the results were found statistically significant (p<0.05). To conclude, our results provide evidences that HTMT, amthamine and clobenpropit (H1-, H2- and H4-agonist, respectively) have important role in modulation of antibody generation by enhancing production level, in which HTMT have dominant role, while amthamine and clobenpropit play similar role. Conversely, R-[-]-?-methylhistamine (H3-agonist) have dominant inhibitory role on antibody production.

Keywords: Histamine, histamine receptors, agonists, immunomodulation, antibody generation, immunotoxic


Trivendra Tripathi, Mohammad Shahid, Adil Raza, Haris M. Khan, Mashiatullah Siddiqui, Abida Malik, Rahat Ali Khan. In vivo comparative immunotoxic study of histamine receptors (H1R, H2R, H3R and H4R)-agonist. Eastern J Med. 2010; 15(2): 48-56


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